Influence of glycosaminoglycans on endogenous DNA synthesis in isolated normal and cancer cell nuclei. Differential effect of heparin |
| |
| Authors: | Kiyoshi Furukawa V.P. Bhavanandan |
| |
| Affiliation: | Department of Biological Chemistry, The M.S. Hershey Medical Center, The Pennsylvania State University, Hershey, PA 17033, U.S.A. |
| |
| Abstract: | The influence of exogenously-added glycosaminoglycans and glycoproteins on DNA synthesis in isolated nuclei, from normal and malignant tissues, was investigated. Heparin stimulated DNA synthesis in normal cell nuclei at concentrations (heparin/DNA (w/w) <0.9) which inhibited DNA synthesis in tumor cell nuclei. At higher concentrations (heparin/DNA (w/w) > 0.9) heparin inhibited DNA synthesis in both normal and tumor cell nuclei. The chondroitin-4 and 6-sulfates, heparan sulfate, cartilage proteoglycan, N-desulfated heparin, and glycophorin caused inhibition of DNA synthesis at all concentrations tested and in all nuclei examined. Hyaluronic acid, dermatan sulfate, keratan sulfate, α1-acid glycoprotein and fetuin had no significant influence on DNA synthesis in isolated nuclei. |
| |
| Keywords: | Glycosaminoglycan DNA synthesis Heparin effect Tumor |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
