菠萝磷转运蛋白1家族基因鉴定及特征分析

磷转运蛋白1(phosphate transporter protein 1, PHT1)家族在植物对磷的吸收及再利用过程中发挥重要作用。该研究对菠萝PHT1基因(AcoPHT1)进行全基因组鉴定,并对基因结构、编码蛋白保守功能域和基因表达进行了分析。结果表明:(1)共鉴定到9个AcoPHT1基因,位于基因组7个连锁群上,所有基因均含有1～3个内含子,内含子相位类型多样。(2)除AcoPHT1.8外,AcoPHT1蛋白均为碱性蛋白,所有蛋白属于亲水性蛋白,且含有10～13个跨膜功能域,均具有保守的PHT1蛋白标签序列GGDYPLSATIxSE,主要定位于叶绿体和细胞质中。(3)AcoPHT1蛋白聚类在单子叶植物组和单双子叶植物混合组中,相对于拟南芥,水稻PHT1与菠萝PHT1相似度更高。(4)AcoPHT1基因启动子区含有P1BS、W-box等与磷吸收和响应胁迫有关的多个顺式作用元件。(5)靶基因预测分析显示,基因AcoPHT1.2、AcoPHT1.8和AcoPHT1.9受多个miRNA调控。(6)AcoPHT1基因表达存在组织特异性和功能冗余性,不同PHT1基因可能在菠萝不同组织或发育阶段发挥作用。该研究结果为菠萝PHT1家族基因的功能鉴定和育种应用奠定理论基础。

Genomic identification and characterization analysis of the phosphate transporter protein 1 family gene in pineapple

Phosphate transporter protein 1(PHT1)family plays pivotal roles in the uptake and re-mobilization of phosphate of plants. In this study, the analysis of whole genome-wide sequence of PHT1 genes in pineapple(Ananas comosus)was conducted, and the gene structure, encoding protein conserved domain and gene expression were further investigated. The results were as follows:(1)Nine PHT1(AcoPHT1)genes of pineapple, which were located in seven linkage groups and contained one to three introns with various intron phase types, were identified.(2)Except for AcoPHT1.8, AcoPHT1 proteins were all basic proteins, and all the AcoPHT1 proteins were hydrophilic and contained 10 to 13 transmembrane domains, which conserved PHT1 protein tag sequence GGDYPLSATIxSE, and was mainly located in chloroplasts and cytoplasm.(3)AcoPHT1 protein clusters were found in monocotyledons group and monocotyledons and dicotyledons mixed group, and compared to Arabidopsis thaliana, AcoPHT1s proteins had higher similarity with rice PHT1 proteins.(4)The promoter region of AcoPHT1 gene contained a large number of P1BS, W-box and other cis-acting elements, which were related to phosphorus absorption and response to stress.(5)Prediction analysis showed that three genes, AcoPHT1.2, AcoPHT1.8 and AcoPHT1.9 were regulated by multiple miRNAs.(6)The expression of AcoPHT1 gene had tissue-specific and functional redundancy, and different PHT1 genes might play roles in different tissues or development stages. The results provide a theoretical reference for functional identification and breeding application of PHT1 family genes in pineapple.