Translationally controlled tumour protein: A protein necessary for potyvirus intracellular multiplication that supports plant infection by unrelated viruses |
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Authors: | Fernanda Prieto Bruckner Patrícia Oliveira Andrade Renan de Souza Cascardo Jean-Franois Lalibert Poliane Alfenas-Zerbini |
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Institution: | Fernanda Prieto Bruckner,Patrícia Oliveira Andrade,Renan de Souza Cascardo,Jean-François Laliberté,Poliane Alfenas-Zerbini |
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Abstract: | The multifunctional protein translationally controlled tumour protein (TCTP) was previously identified as necessary for infection by the potyvirus pepper yellow mosaic virus. Using turnip mosaic virus (TuMV) as a model to study potyvirus biology, we confirmed that TCTP has a positive effect on virus infection. Living cell confocal microscopy demonstrated that TCTP colocalises with 6K2-tagged replication vesicles and with a perinuclear globular structure typically observed during potyvirus infection. Also, TCTP silenced protoplasts showed reduced virus accumulation, quantified by qRT-PCR, which suggests an effect on virus replication, translation or other intracellular process. Finally, TCTP silencing in plants reduced the accumulation of two species belonging to Orthotospovirus and a Begomovirus genus, which are not closely related to potyviruses. The results suggest that TCTP is a general susceptibility factor to several unrelated viruses. |
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Keywords: | begomovirus orthotospovirus plant– virus interaction translationally controlled tumour protein turnip mosaic virus virus replication |
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