周期性张应力刺激下成肌细胞钙网蛋白的表达及变化

目的：检测成肌细胞钙网蛋白（CRT）在循环拉伸应力刺激下的表达变化。方法：体外构建面颌部成肌细胞力学刺激模型。加力组以0．5赫兹的加载频率和10％细胞拉伸变形幅度对细胞进行加力培养1h，6h，12h，24h，运用实时荧光定量PCR技术跟Westem Blot技术分别检测在周期性张应力作用下成肌细胞CRT在基因水平及蛋白水平的表达变化。结果：当对细胞加力6h后，CRTmRNA及蛋白表达量开始增多，加力12h后CRTmRNA及蛋白表达量到达最多（P〈0．01），加力0h组与加力12h组之间差异有显著的统计学意义（P〈0．01）。结论：持续的周期性张应力刺激下CRTmRNA及蛋白表达增加。

Expression and Change of CRT in Stretch -induced Apoptosis of Myoblast

Objective： To investigate the changes of Calcium protein （CRT） expression in myoblast under cyclic tensile stress stimulus. Methods： The culture-tensile stimulate model of myoblast in vitro was established. The strength group cells were cultured for 1 h, 6 h, 12 h, 24 h under 0.5 hz loading frequency and 10% tensile deformation amplitude. PCR and Western Blot technique was used to detect the changes of CRT in the genetic level and protein level under cyclic tensile stress. Results： After 6 h cyclic tensile stress, the expression ofCRT mRNA and protein began to increase, after 12 h cyclic tensile stress, the expression ofCRT mRNA and protein reached a maximum （P〈0.01）, the difference between 0 h group and 12 h has obvious statistical significance （P〈0.01）. Conclusions： The expression of CRT mRNA and protein can increase under continuous tensile stress stimulation.