白念珠菌基因分型的相关研究

白色念珠菌定植于大多数人群的口腔中，在一定条件下可成为优势菌种而导致感染。基因分型是近年来白念分子生物学研究中的一个热点，随着医学科学技术的发展，由于深部真菌感染比例不断增加，分子生物学方法已经越来越广泛的应用于临床真菌病的研究中，从而为控制白念感染及为早期诊断、治疗提供基础。本文综述了限制性片段长度多态性、随机扩增多态性DNA分析、ITS区域序列分析等分子生物学技术在白念珠菌基因分型方面的相关研究，比较了它们的优缺点，并且讨论了将基因分型研究应用于临床诊断、治疗及开发新型抗真菌药物的发展趋势和广阔前景。认为目前更倾向于多种分型方法联合应用，并借助计算机软件进行分析，但是仍需进一步探索。

Related Research of Candida Albicans Genotyping

Candida albicans colonization in the mouth of the majority of the population, under certain conditions, can become the dominant strains and lead to infection. In recent years,geno typing is a hot topic in molecular biology researching of albicans, with the development of science and technology, because of deep fungal infection ratio increases unceasingly, molecular biology method has been more and more widely used in clinical fungal disease research, so as to control albicans infection and provide a basis for the early diagnosis and treatment. This paper reviews theRFLP, RAPD, ITS regional sequence analysis of molecular biology technology in c. albicans genotyping related research, compared their advantages and disadvantages, and discuss the genotyped research applied to clinical diagnosis, treatment and the development of new antifungal development trend and board prospects. We believe that it is more useful to a variety of typing methods in combination, and with the aid of computer software to carry on the analysis, but it still need further investigation.