Structural Proteins of Adenovirus-Associated Viruses |
| |
Authors: | J. A. Rose J. V. Maizel Jr. J. K. Inman A. J. Shatkin |
| |
Affiliation: | National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014;Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461 |
| |
Abstract: | The structural proteins of adenovirus-associated virus (AAV) types 1, 2, and 3 were analyzed by acrylamide gel electrophoresis. In each case, one major protein (C) and two minor proteins (A and B) were identified. Component C had an estimated molecular weight of 62,000 daltons, and the molecular weights of components A and B were found to be 87,000 and 73,000 daltons, respectively. Coelectrophoresis of adenovirus and AAV proteins revealed an overlap only between the adenovirus fiber-penton component and the AAV C polypeptide. Among AAV serotypes, homologous components were electrophoretically identical, except that the C component of AAV-2 was of slightly lower molecular weight than the C components of AAV-1 and AAV-3. The relative incorporation of (14)C-arginine and (14)C-mixed amino acids into the three polypeptides of AAV-2 was similar, indicating an absence of an arginine-rich component. In addition, AAV-2 was found to have a substantially lower arginine content than helper adenoviruses. |
| |
Keywords: | |
|
|