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Proteomics Analysis of Extracellular Matrix Remodeling During Zebrafish Heart Regeneration
Affiliation:3. 3Center of Regenerative Medicine in Barcelona (CMRB), 3rd Floor Hospital Duran i Reynals, Avinguda de la Gran Via 199–203, 08908 Hospitalet de Llobregat (Barcelona), Spain;4. 4Center for Networked Biomedical Research on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 08908 Hospitalet de Llobregat (Barcelona), Spain;5. 5Bioinformatics Unit, Institut d''Investigació Biomèdica de Bellvitge IDIBELL), 3rd Floor Hospital Duran i Reynals, Avinguda de la Gran Via 199–203, 08908 Hospitalet de Llobregat (Barcelona), Spain;6. 6Institute for Bioengineering of Catalonia (IBEC), Barcelona Science Park, Baldiri Reixac 15–21, 08028 Barcelona, Spain;3. 12Center for Networked Biomedical Research on Respiratory Diseases (CIBERES), 08036 Barcelona, Spain;8. 8Proteomics group, Vall d''Hebron Institut of Oncology (VHIO), Cellex center, Natzaret 115–117, 08035 Barcelona, Spain;9. 9Institució Catalana de Recerca i Estudis Avançats (ICREA), 08010 Barcelona, Spain
Abstract:
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  • Highlights
    • •We have developed a decellularization protocol for ECM protein enrichment.
    • •We have characterized the proteome of adult zebrafish heart ECM.
    • •We describe dynamic changes in heart ECM proteome during regeneration.
    • •We describe changes in heart ECM stiffness during regeneration.
    Keywords:Extracellular matrix  Cardiovascular function or biology  Animal models  Developmental biology  Cardiovascular disease  atomic force microscopy  Extracellular matrixs  heart regeneration  proteomic analysis
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