Disc Electrophoretic Studies on an S-Carboxymethylkerateine Preparation from Bovine and Canine Hair

Reduced and iodoacetate alkylated keratin, S-carboxymethyl-kerateine (SCMK), may be separated in components differing in molecular weight and sulphur content (Gillespie et al. 1962). Species differentiation could be accomplished by moving boundary electrophoresis of high-sulphur kerateines, $${text{S}}_{20,{text{w}}}^{text{O}}$$ 1.55-1.65 (Gillespie 1963, Gillespie & Inglis 1965). Shechter et al. (1969) observed species differences in the acrylamide electrophero-grams of low-sulphur kerateines, mol. w. 45,000-50,000.