Addition of procyanidine to semen preserves progressive sperm motility up to three hours of incubation |
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| Institution: | 1. Unit for Human Reproduction, Department of Obstetrics and Gynecology, Medical School, Sohag University Hospital, Sohag, Egypt;2. Unit for Human Reproduction, 1st Department of Obstetrics and Gynecology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece;1. Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China;2. Key Laboratory of Birth Regulation and Control Technology of National Health and Family Planning Commission of China, Jinan, Shandong, China;3. Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China;4. National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong, China;5. The Key laboratory for Reproductive Endocrinology of Ministry of Education, Shandong, China;6. Maternal and Child Health Care of Shandong Province, Jinan, Shandong, China;1. VID Specialized University, Faculty of Health studies, Oslo, Norway;2. Faculty of Veterinary Medicine, IRAS, Utrecht University, Utrecht, the Netherlands;3. Department of Environment and Health, Faculty of Science, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands;4. New Children’s Hospital, Pediatric Research Center, University of Helsinki and Helsinki University Hospital, Helsinki, Finland;5. Department of Women’s and Children´s Health, NORDFERTIL Research Lab, Stockholm, Sweden;6. Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Castanhal, Pará, Brazil;7. Schothorst Feed Research, Lelystad, the Netherlands;8. The production animal experimental research centre (SHF), Norwegian University of Life Sciences, Oslo, Norway;1. Departamento de Ciências Naturais, Universidade Federal de São João del Rei. Praça Dom Helvécio, 74 - Dom Bosco, São João del-Rei, MG, 36301-160, Brazil;2. Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, Belo Horizonte, MG, 31270-901, Brazil;3. Departamento de Zootecnia, ESALQ/Universidade de São Paulo, Av. Pádua Dias 11, Piracicaba, SP, 13418-900, Brazil;4. Embrapa Recursos Geneticos e Biotecnologia, PqEB, Av. W5 Norte, Brasília, DF, 70770-917, Brazil;1. Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, PR China;2. Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, 075000, PR China;3. Department of Oncology, People Hospital Affiliated to Hebei Medical University, Shijiazhuang, 050000, PR China;1. Faculty of Medicine, Universiti Teknologi MARA Sungai Buloh Campus, Selangor, Malaysia;2. I-PPerFORM, Universiti Teknologi MARA, Malaysia |
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| Abstract: | Several studies on semen physiology and sperm fertilizing capacity have shown a beneficial effect of antioxidants. Procyanidine is a natural antioxidant, more efficient compared with vitamin C and E, with many applications in the food, agriculture, pharmaceutical and cosmetic industry. Thus, we tested whether the addition of procyanidine to the semen of infertile men has a beneficial effect on spermatozoa during their in vitro incubation and during the cryopreservation process. Semen samples of 25 infertile men were divided in to two aliquots, in which procyanidine was added or not. Semen analysis, measurement of sperm DNA fragmentation index (DFI) and measurement of reactive oxygen species (ROS) were performed 3 h after incubation at 37 °C and after sperm cryopreservation and thawing. In-vitro addition of procyanidine to semen of infertile men resulted in a lesser decrease in progressive motility −4 (−31:+6) vs. −6 (−31:+5), p < 0.001] and total motility −5 (−29:+3) vs. −9 (−32:+2), p < 0.001] after 3 h of incubation compared with no addition of procyanidine. Sperm morphology was decreased only in the control group after 3 h of incubation 2 (0:+6) vs. 1 (0:+4), p = 0.009]. Furthermore, a larger increase in sperm DFI was observed in the control compared with the procyanidine group 9 (−7:+27) vs. 3 (−3:+18), p = 0.005] after thawing of cryopreserved semen samples. In conclusion, in-vitro addition of procyanidine to the semen of infertile men exerts a protective effect on progressive motility during handling and after 3 h of incubation as well as on sperm DFI during the process of cryopreservation. |
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| Keywords: | Semen analysis Procyanidine DNA fragmentation index Cryopreservation Antioxidants |
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