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Integrated Analyses of Phenotype and Quantitative Proteome of CMTM4 Deficient Mice Reveal Its Association with Male Fertility
Institution:From the 3Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Key Laboratory of Medical Immunology (Ministry of Health), Peking University Center for Human Disease Genomics, Beijing, 100191, China;;4Department of Central Laboratory, Affiliated Yantai Yuhuangding Hospital of Qingdao University, Shandong Province, 264000, China;;5Shandong Research Centre for Stem Cell Engineering, Affiliated Yantai Yuhuangding Hospital of Qingdao University, Shandong Province, 264000, China;;6Reproduction Medical Center, Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, Shandong, P.R. China
Abstract:
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  • Highlights
    • ?CMTM4 is associated with human spermatogenesis and sperm quality.
    • ?Cmtm4 knockout mouse were generated by CRISPR/Cas9 technology for male fertility research.
    • ?CMTM4 is required for male fertility but not female fertility.
    • ?Phenotype and quantitative proteomics of Cmtm4 KO mice reveal an association of CMTM4 with histone-to-protamine exchange, sperm motility and induction of the acrosome reaction.
    Keywords:Cytokines*  iTRAQ  Knockouts*  Mouse models  Absolute quantification  Cas9  CMTM4  Male fertility  Spermatogenesis  Testis
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