Investigating Lactococcus lactis MG1363 Response to Phage p2 Infection at the Proteome Level期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Investigating Lactococcus lactis MG1363 Response to Phage p2 Infection at the Proteome Level

Institution:	3. From the 3Département de biochimie, de microbiologie, et de bio-informatique, Faculté des sciences et de génie, Université Laval, Québec City, QC, G1V 0A6, Canada;;4. 4Groupe de recherche en écologie buccale, Faculté de médecine dentaire, Université Laval, Québec City, QC, G1V 0A6, Canada;;5. 5Institute of Microbiology, University of Greifswald, Greifswald, Germany;;6. Félix d''Hérelle Reference Center for Bacterial Viruses, Faculté de médecine dentaire, Université Laval, Québec City, QC, G1V 0A6, Canada

Abstract:	Download : Download high-res image (89KB) Download : Download full-size image Highlights ?The proteomes of L. lactis MG1363 and phage p2 at different stages of infection were characterized. ?16% (226/1412) of the bacterial proteins detected were unique to infected cultures. ?A targeted approach using synthetic peptides improved the coverage of phage p2 proteome. ?By means of proteogenomics, we uncovered a conserved phage protein coded by a previously unannotated gene. ?Deletion of the bacterial gene llmg_0219 (unknown function) impedes phage p2 infection.

Keywords:	Mass Spectrometry Label-free quantification Bacteria Viruses Proteogenomics Genome editing Lactococcus lactis Phage infection
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