The Suitability of Some Media and Peptones for Sulfonamide Testing

GUDDING, ROAR: The suitability of some media and peptones for sulfonamide testing. Acta vet. scand. 1974, 15, 366–380. — The content of p-aminobenzoic acid and folic acid has been determined in some media and peptones, and the sensitivity of Bacillus megaterium, cultivated on these substrates, towards sulfonamides has been examined. The concentrations of p-aminobenzoic acid and folic acid should be as low as possible in suitable substrates, but other factors are also of importance. The presence of antagonists towards folic acid has been demonstrated in some media, and the consequences of these inhibitors are discussed. Three of the media (Paper Disc Method-Antibiotic Sensitivity Medium, AB Biodisk; Sulphonamide Antagonist Free Medium, Mast Laboratories; and Mueller Hinton, Difco Laboratories) seem to be satisfactory for the determination of the resistance of bacteria towards sulfonamides. The application of Acetobacter medium is suggested for the detection of rest concentrations of sulfonamides in foodstuffs. detection of sulfonamide residues; p-aminobenzoic acid; folic acid.