Indirect Fluorescent Antibody Techniques for Demonstration of Trout Viruses and Corresponding Antibody |
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| Authors: | Vestergárd Jørgensen P. E. |
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| Affiliation: | 1.Department for Jutland, State Veterinary Serum Laboratory, Hangøvej 2, Arhus, DK-8200, Denmark
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| Abstract: | Two variations of the indirect fluorescent antibody technique (FAT) have been utilized in the work concerning two important virus diseases of trout, viral haemorrhagic septicaemia (VHS) and infectious pancreatic necrosis (IPN). A “two layer” indirect FAT allowed demonstration of the respective viruses in cell cultures and a “three layer” indirect FAT allowed demonstration of trout antibody to the viruses. Antibody, by means of the latter technique, could be demonstrated only in artificially immunized trout. |
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