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Data-independent Acquisition Improves Quantitative Cross-linking Mass Spectrometry

Institution:	3. From the 3Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany;;4. 4Wellcome Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, Scotland, United Kingdom;;5. 5Biognosys, 8952 Zürich-Schlieren, Switzerland

Abstract:	Download : Download high-res image (126KB) Download : Download full-size image Highlights •Quantitative cross-linking mass spectrometry (QCLMS) was automated by Spectronaut. •Data-independent acquisition (DIA) was adapted to QCLMS. •Accuracy and precision of quantitation improves with DIA over DDA. •QCLMS is now ready for use in complex samples.

Keywords:	Protein Cross-linking* Label-free quantification Mass Spectrometry Quantification Bioinformatics software Data independent acquisition Reproducibility Spectronaut Xi
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