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Whole Proteome Profiling of N-Myristoyltransferase Activity and Inhibition Using Sortase A

Institution:	3. From the 3Department of Chemistry, Imperial College London, White City Campus, 80 Wood Lane, London W12 0BZ, UK.

Abstract:	Download : Download high-res image (114KB) Download : Download full-size image Highlights ?Application of Sortase A to label protein N-termini across the whole proteome. ?Novel gel, proteomic and ELISA-based methods to determine N-myristoylation of proteins in cells, without metabolic labelling. ?Side by side mass spectrometric quantification of changes in protein N-myristoylation by two complementary methods. ?Improved Biotin-Neutravidin affinity enrichment protocol.

Keywords:	Chemical Biology Drug Targets N-terminal Modifications Substrate Identification Tandem Mass Spectrometry IMP-1088 N-Myristoylation NMT Inhibitor Sortase A
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