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Proteome-wide Analysis of Protein Thermal Stability in the Model Higher Plant Arabidopsis thaliana
Affiliation:3. Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706;;4. Biomolecular Mass Spectrometry and Proteomics, Utrecht University, Utrecht, Netherlands
Abstract:
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  • Highlights
    • •Over 1700 Arabidopsis proteins with thermal models in multiple replicates.
    • •Melting temperature correlates with 1°, 2°, and 3° protein characteristics.
    • •Ligand-induced thermal shifts are evident in complex protein extracts.
    Keywords:Protein Conformation  Plant Biology  Tandem Mass Spectrometry  Arabidopsis  Quantification  Thermal Proteome Profiling
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