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Quantitative Proteomics of Uukuniemi Virus-host Cell Interactions Reveals GBF1 as Proviral Host Factor for Phleboviruses
Institution:3. CellNetworks Cluster of Excellence and Department of Infectious Diseases, Virology, University Hospital Heidelberg, Heidelberg, Germany;4. Institute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hannover, Germany;5. Experimental Systems Immunology, Max Planck Institute of Biochemistry, Martinsried, Germany;6. Division of Virology, Department of Clinical Microbiology, and Laboratory for Molecular Infection Medicine Sweden, Umeå University, Umeå, Sweden;12. Institute of Virology, Charité, Universitätsmedizin Berlin, Berlin, Germany and Berlin Institute of Health (BIH), Berlin, Germany;8. IVPC UMR754, INRA, Univ. Lyon, EPHE, 50 Av. Tony Garnier, 69007 Lyon, France;9. Department of Clinical Microbiology, Virology & Wallenberg Centre for Molecular Medicine (WCMM), Umeå University, SE-90185 Umeå, Sweden
Abstract:
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  • Highlights
    • ?Quantitative proteomics reveals GBF1 to interact with Uukuniemi virus glycoproteins.
    • ?GBF1 is a proviral host factor for phleboviruses and other zoonotic RNA viruses.
    • ?GBF1 supports phlebovirus replication and assembly and release of particles.
    • ?The GBF1 inhibitor Golgicide A blocks infection with zoonotic RNA viruses.
    Keywords:Viruses  glycoproteins  affinity proteomics  label-free quantification  peptide mass fingerprinting  assembly  egress  GBF1  replication  Uukuniemi
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