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Proteomic Profiling of Human Prostate Cancer-associated Fibroblasts (CAF) Reveals LOXL2-dependent Regulation of the Tumor Microenvironment
Affiliation:From the 3Cancer Program, Biomedicine Discovery Institute,;Departments of 4Biochemistry and Molecular Biology,;5Anatomy and Developmental Biology, and;123Physiology, Monash University, Clayton, Australia;;6Cancer Research Division, Peter MacCallum Cancer Centre, Victorian Comprehensive Cancer Centre, Parkville, Australia;;**School of Health and Wellbeing, University of Southern Queensland, Ipswich, Queensland, Australia;;12Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Center for Cancer Bioinformatics, Peking University Cancer Hospital & Institute, Beijing, China;;8The Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Sydney, Australia;;9St Vincent''s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, Australia;;10Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Australia
Abstract:
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  • Highlights
    • •Quantitative (phoshpo)proteome of primary cell cultures of patient-matched prostate CAF and NPF.
    • •Key CAF-associated proteins validated using orthogonal methodologies.
    • •LOXL2 inhibitors D-penicillamine and PXS-S2A impaired CAF migration and ECM alignment.
    • •Pre-treatment with LOXL2 inhibitors impaired migratory capacity of RWPE-2 cells in co-culture.
    Keywords:Phosphoproteome  Prostate cancer  Prostate cancer biomarkers  Tumor microenvironment*  Cancer biomarker(s)  Cancer-associated fibroblasts  Fibroblasts  LOXL2  Non-malignant prostate fibroblasts
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