Sample Preparation Scale-Up for Deep N-glycomic Analysis of Human Serum by Capillary Electrophoresis and CE-ESI-MS*期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Sample Preparation Scale-Up for Deep N-glycomic Analysis of Human Serum by Capillary Electrophoresis and CE-ESI-MS*

Institution:	3. MTA-PE Translational Glycomics Research Group, Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, 10 Egyetem Street, Veszprem, 8200, Hungary;4. Horváth Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, 98 Nagyerdei krt., Debrecen, 4032, Hungary

Abstract:	Download : Download high-res image (84KB) Download : Download full-size image Highlights •Efficient sample preparation workflow for deep N-glycomics analysis from serum. •Temperature gradient denaturing protocol to prevent protein precipitation. •Decrease of free sugar content in serum enhanced PNGase F digestion efficiency. •Modified evaporative labeling method increased fluorophore labeling yield.

Keywords:	Glycomics separation technologies mass spectrometry electrophoresis serum/plasma capillary electrophoresis CE-MS
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