A comparative study of ryanodine receptor (RyR) gene expression levels in a basal ray-finned fish, bichir (Polypterus ornatipinnis) and the derived euteleost zebrafish (Danio rerio) |
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Authors: | Siavash Darbandi Jens P.C. Franck |
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Affiliation: | aDepartment of Biology, University of Winnipeg, 515 Portage Ave., Winnipeg, Canada MB R3B 2E9;bDepartment of Biochemistry and Medical Genetics, University of Manitoba, 745 Bannatyne Ave., Winnipeg, Canada MB R3E 0J9 |
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Abstract: | Ryanodine receptors (RyRs) are large homotetrameric protein complexes that mediate the release of intracellular stores of calcium. Mammals possess three gene copies, RyR1, RyR2, and RyR3 that are expressed in a variety of tissue types. Teleost fish express RyR1a and RyR1b genes that are expressed in slow twitch skeletal muscle and fast twitch skeletal muscles respectively. Here we report the results of a survey of the genome of bichir (Polypterus ornatipinnis), considered the most basal ray-finned fish, for its RyR genes. The bichir genome encodes four RyR genes, RyR1a, RyR1b, RyR2, and RyR3 that phylogenetically cluster with their vertebrate orthologs. Quantitative real time PCR shows fibre type-specific expression of the RyR1a and RyR1b genes. The RyR3 gene, however, is down regulated in bichir in contrast to derived teleosts including zebrafish in which the RyR1 and RyR3 genes are co-expressed at equivalent levels. |
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Keywords: | Ryanodine receptor Genome duplication qRT-PCR Molecular evolution Gene expression |
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