Neurite-promoting factors from a sympathetically innervated target tissue |
| |
Authors: | Wilson P. A. Dortmans M. Rush R. A. |
| |
Affiliation: | (1) Center for Neuroscience School of Medicine, Flinders University of South Australia, 5042 Bedford Park, South Australia;(2) Unit of Human Physiology School of Medicine, Flinders University of South Australia, 5042 Bedford Park, South Australia |
| |
Abstract: | Previous studies have demonstrated that various cell types can produce and secrete polyornithine-attachable neurite promoting factors when cultured. This study describes an endogenous source of polyornithine-attachable neurite promoting factors. The active material extracted from an avian smooth muscle, the expansor secundariorum, is able to enhance neurite outgrowth from embryonic chick sympathetic neurons when applied to a polyornithine substrate. Unlike other polyornithine attachable factors, the material is also able to support the neurons for at least 72 hr in the absence of any added survival factors. Partial purification of the active material was achieved by affinity chromatography on polyornithine-Sepharose. The findings support the proposal that neurite promoting factors may have a definite physiological role in addition to their well established in vitro activity. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|