Differential Effect of Cd2+ and Ni2+ on Amino Acid Metabolism in Soybean Seedlings |
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Authors: | F El-Shintinawy A El-Ansary |
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Institution: | (1) Botany Department, Faculty of Science, Tanta University, Tanta, Egypt E-mail;(2) Laboratory of Medicinal Chemistry, National Research Center, Cairo, Egypt |
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Abstract: | In 10-d-old soybean seedlings, the growth of roots and shoots was significantly inhibited at 50 and 100 M and more Cd2+, respectively, and by 50 M or more Ni2+. Although total protein content of roots exposed to 200 M Cd2+ or Ni2+ was similarly decreased compared to the control, the activity of nitrate reductase was much more inhibited by Cd2+. Ni2+-treatment (200 M) induced an accumulation of all free amino acids in roots associated with a decrease in alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities reflecting the accumulation of both alanine and aspartic acid, respectively. Cd2+-treatment (200 M) decreased the amount of all free amino acids. In addition, cysteine which is the main amino acid consisting the phytochelatin complexes constituted about 17.5 % of total free amino acids. The activities of both ALT and AST in Cd2+-treated roots were higher than in Ni2+-treated roots suggesting higher conversion of alanine and aspartate to pyruvate and oxaloacetate. Primary leaves excised from either Cd2+ or Ni2+-treated seedlings showed similar pattern of enzyme activities as roots. |
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Keywords: | alanine aminotransferase aspartate aminotransferase cysteine Glycine max heavy metals HPLC nitrate assimilation |
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