Characterization of glucose oxidase-negative mutants of a lignin degrading basidiomycete Phanerochaete chrysosporium |
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Authors: | R L Kelley K Ramasamy C Adinarayana Reddy |
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Institution: | (1) Department of Microbiology and Public Health, Michigan State University, 48824-1101 East Lansing, MI, USA |
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Abstract: | The isolation and characterization of glucose oxidase-negative (gox
-) mutants of Phanerochaete chrysosporium, is described. These mutants are deficient not only in their ability to produce hydrogen peroxide (H2O2) but also in lignin degradation (2-14C-synthetic lignin14CO2), ligninase and peroxidase activities, decolorization of the dye poly-R 481, and production of ethylene from -oxo--methylthiobutyric acid (KTBA). The gox
- mutants retained, albeit at a lower level, the capacity to produce veratryl alcohol, a typical secondary metabolite, and produced conidia at a level comparable to that of the wild type. The addition of ligninase and/or glucose oxidase to a gox
- mutant (GOX-10) did not enhance its capacity to degrade lignin. The Gox+ revertant strains regained glucose oxidase activity, the ability to degrade lignin, as well as the other characteristics that were missing in the gox
- mutants. The results suggest that the genetic lesion in these mutants affects the regulation of a set of secondary metabolic characteristics.Abbreviations Gox
glucose oxidase
- KTBA
-oxo--methylthiobutyric acid
Journal article no. 11740 from the Michigan Agricultural Experiment Station |
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Keywords: | Glucose oxidase Lignin degradation Phanerochaete chrysosporium Lignin degrading basidiomycete Glucose oxidase-negative mutants gox mutants Ligninase Peroxidase -Oxo-gif" alt="agr" align="BASELINE" BORDER="0">-Oxo--methylthiobutyric acid" target="_blank">gif" alt="gamma" align="MIDDLE" BORDER="0">-methylthiobutyric acid 14C-Synthetic lignin |
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