An indirect role of vitamin B12 in regulation of thymidylate synthase in Lactobacillus leichmannii. |
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Authors: | C P Nair J M Noronha |
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Institution: | Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India. |
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Abstract: | Vitamin B12 augments thymidylate synthase function in L. leichmannii by facilitating indirectly the availability of suitable nonmethylpolyglutamylfolate cofactors. This is effected by the demethylation of trapped methyltetrahydrofolates, catalysed by a vitamin B12 requiring methionine synthase. Deoxyuridine supplemented cells, lacking in B12, have decreased levels of methionine synthase and thymidylate synthase. Addition of active and inactive conjugase preparation as a source of mono and polyglutamylfolates indicated that the latter are the preferred cofactors for thymidylate synthase. |
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