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Cellulose-bound Peptide Arrays: Preparation and Applications |
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| Authors: | Kai Hilpert Dirk FH Winkler Robert EW Hancock |
| Institution: | 1. Centre for Microbial Diseases and Immunity Research, University of British Columbia , 2259 Lower Mall Research Station, Vancouver, British Columbia , V6T 1Z3 , Canada bob@cmdr.ubc.ca;3. Peptide Array Facility of the Brain Research Centre, University of British Columbia , 2211 Westbrook Mall, Vancouver , British Columbia , V6T 2B5 , Canada;4. Centre for Microbial Diseases and Immunity Research, University of British Columbia , 2259 Lower Mall Research Station, Vancouver, British Columbia , V6T 1Z3 , Canada |
| Abstract: | Spatial organization of metabolic enzymes may represent a general cellular mechanism to regulate metabolic flux. One recent example of this type of cellular phenomenon is the purinosome, a newly discovered multi-enzyme metabolic assembly that includes all of the enzymes within the de novo purine biosynthetic pathway. Our understanding of the components and regulation of purinosomes has significantly grown in recent years. This paper reviews the purine de novo biosynthesis pathway and its regulation, and presents the evidence supporting the purinosome assembly and disassembly processes under the control of G-protein-coupled receptor (GPCR) signaling. This paper also discusses the implications of purinosome and GPCR regulation in drug discovery. |
| Keywords: | drug discovery dynamic mass redistribution assay G protein-coupled receptor metabolic flux mitogenic signaling purine de novo biosynthesis purine salvage pathway purinosome |