Enzymatic,outer membrane proteins and plasmid alterations of starved <Emphasis Type="Italic">Vibrio parahaemolyticus</Emphasis> and <Emphasis Type="Italic">Vibrio alginolyticus</Emphasis> cells in seawater |
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Authors: | Fethi Ben Abdallah Héla Kallel Amina Bakhrouf |
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Institution: | (1) Laboratoire d’Analyse, Traitement et Valorisation des Polluants de l’Environnement et des Produits, Faculté de Pharmacie Rue Avicenne, 5000 Monastir, Tunisia;(2) Unité de Fermentation et de Développement de Vaccins Virologiques, Institut Pasteur de Tunis, 13 Place Pasteur, 1002 Tunis, Tunisia |
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Abstract: | The marine bacteria Vibrio parahaemolyticus and V. alginolyticus were incubated in seawater for 8 months to evaluate their adaptative responses to starvation. The starved cells showed an
altered biochemical and enzymatic profiles, respectively, on Api 20E and Api ZYM systems and an evolution to the filterable
minicells state capable to pass membrane pore size 0.45 μm. Outer membrane proteins patterns of stressed bacteria were also
altered. Indeed, these modifications were manifested by the appearance and/or disappearance of bands as well as in the level
of expression of certain proteins. Plasmids profiles analysis showed that V. alginolyticus ATCC 33787 lost three plasmids, whereas other tested strains conserved their initial profiles. |
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Keywords: | Vibrio Seawater Starvation Enzymatic Morphological Outer membrane proteins Plasmids Alterations |
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