Osteopontin promotes hepatocellular carcinoma invasion by up-regulating MMP-2 and uPA expression |
| |
Authors: | Rong-Xin Chen Yun-Hong Xia Tong-Chun Xue Sheng-Long Ye |
| |
Institution: | (1) Liver Cancer Institute and Zhongshan Hospital, Fudan University, 200032 Shanghai, China;(2) Key Laboratory for Carcinogenesis and Cancer Invasion, Chinese Ministry of Education, 200032 Shanghai, China; |
| |
Abstract: | Osteopontin (OPN) is over-expressed in a variety of cancers, but its role in hepatocellular carcinoma (HCC) progression has
not been clarified. In this study, weakly tumorigenic, non-metastastic human HCC cell line SMMC-7721 cells were forced to
over-express OPN via stable transfection. A series of functional assays were performed to assess the effects of OPN on tumor
cell behaviors and cDNA microarray was used to identify the genes regulated by OPN. The results showed that OPN significantly
enhanced the migration and invasion of SMMC-7721 cells in vitro. In addition, CD44v6 antibody could significantly inhibit the invasion of OPN over-expressing SMMC-7721 cells. Moreover, MMP-2 and uPA expressions
were significantly up-regulated in OPN over-expressing SMMC-7721 cells. Together, these findings indicate that OPN enhanced
HCC cells invasion through interaction with its receptor CD44v6 and increased MMP-2 and uPA expressions, providing at least
one mechanism for OPN-mediated HCC progression and metastasis. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|