Genetic characterization of a new set of recombinant inbred lines (LGXSM) formed from the intercross of SM/J and LG/J inbred mouse strains |
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Authors: | Tomas Hrbek Reinaldo Alves de Brito B Wang L Susan Pletscher James M Cheverud |
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Institution: | 1. Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 S. Euclid Ave., St. Louis, Missouri, 63110, USA 2. Departamento de Biologia Geral – ICB, Universidade Federal de S?o Carlos, S?o Carlos, S?o Paulo, Brazil
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Abstract: | A new set of LGXSM recombinant inbred (RI) strains is presented. The RI strain panel consists of 18 remaining strains of the
original 55 founding strains. Strain characterization is based on 506 polymorphic microsatellites and 4289 single nucleotide
polymorphisms (SNPs) distributed across the genome. Average microsatellite intermarker distance is 4.80 ± 4.84 Mb or 2.91
± 3.21 F2 cM. SNPs are more densely spaced at 0.57 ± 1.27 Mb. Ninety-five percent of all microsatellite intermarker intervals are separated
by less than 15.00 Mb or 8.50 F2 cM, while 95% of the SNPs are less than 0.95 Mb apart. Strains show expected low levels of nonsyntenic association among
loci and complete genomic independence. During inbreeding, the RI strains went through strong natural selection on the agouti locus on Chromosome 2, especially when the epistatically interacting tyrosinase locus on Chromosome 7 carried the wild-type
allele. The LG/J and SM/J strains differ in a large number of biomedically important traits, and they and their intercross
progeny have been used in multiple mapping studies. The LG×SM RI strain panel provides a powerful new resource for mapping
the genetic bases of complex traits and should prove to be of great biomedical utility in modeling complex human diseases
such as obesity and diabetes.
Electronic Supplementary Material Electronic Supplementary material is available for this article at
and accessible for authorised users. |
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