Mode of reproduction is detected by Parth1 and Sex1 SCAR markers in a wide range of facultative apomictic Kentucky bluegrass varieties |
| |
Authors: | Albertini Emidio Barcaccia Gianni Porceddu Andrea Sorbolini Silvia Falcinelli Mario |
| |
Affiliation: | (1) Present address: Dipartimento di Agronomia Ambientale e Produzioni Vegetali, University of Padova, Agripolis, Via Romea 16, 35020 Legnaro, Padova, Italy;(2) Dipartimento di Biologia Vegetale e Biotecnologie Agroambientali, University of Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy |
| |
Abstract: | Gametophytic apomixis in Kentucky bluegrass (Poa pratensis L.) involves the parthenogenetic development of unreduced eggs from aposporic embryo sacs. Marker-assisted selection for the mode of reproduction in P. pratensis would avoid costly and time-consuming phenotypic progeny tests. We developed and tested two SCAR primer pairs that are associated with the mode of reproduction in P. pratensis. The SCAR primers identified the apomictic and sexual genotypes among progenies of sexual x apomictic crosses with very low bias. Furthermore, when tested on a wide range of Italian and exotic P. pratensis germplasm, they were able to unequivocally distinguish sexual from apomictic genotypes. This system should, therefore, allow new selection models to be set up in this species. |
| |
Keywords: | AFLP Apomixis Marker-assisted selection Poa pratensis L. SCAR |
本文献已被 SpringerLink 等数据库收录! |
|