Electrophoresis karyotype and chromosome-length polymorphism of Histoplasma capsulatum clinical isolates from Latin America |
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Authors: | Canteros Cristina Elena Zuiani María Fernanda Ritacco Viviana Perrotta Diego E Reyes-Montes María Rocío Granados Julio Zúñiga Gerardo Taylor Maria Lucia Davel Graciela |
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Affiliation: | Departamento Micología, INEI ANLIS "Dr. Carlos G. Malbrán", Av. Velez Sarsfield 563, 1281 Buenos Aires, Argentina;Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina;Departamento de Microbiología-Parasitología, Facultad de Medicina, Universidad Nacional Autónoma México, México DF, 04510, Mexico;Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán", México DF, 14000, Mexico;Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México DF, 11340, Mexico |
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Abstract: | Intact chromosomes of 19 clinical isolates of Histoplasma capsulatum recently obtained in Argentina, Mexico and Guatemala and the laboratory reference strain G186B from Panama were analyzed using pulsed-field gel electrophoresis. Chromosomal banding patterns of the human isolates revealed 5-7 bands, ranging from 1.3 to 10 Mbp in size. Strain G186B showed five bands of approximately 1.1, 2.8, 3.3, 5.4 and 9.7 Mbp. Thirteen different electrokaryotypes were identified, indicating that the genome of H. capsulatum varies widely in nature, as observed previously in laboratory strains. No definite association was found between electrokaryotype and geographical or clinical source. |
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Keywords: | Histoplasma capsulatum Karyotyping Pulsed-field gel electrophoresis Chromosomes |
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