Chromosome Replication in Toluenized Bacillus subtilis Cells |
| |
Authors: | TATSUO MATSUSHITA KALPANA P. WHITE NOBORU SUEOKA |
| |
Affiliation: | 1.Department of Biochemical Sciences,Princeton University,Princeton |
| |
Abstract: | BIOCHEMICAL studies of chromosome replication have been hampered by the unavailability of an adequate in vitro system with the basic features of in vivo DNA replication. The criteria for such a system are: (1) semiconservative replication; (2) normal biological activity of newly synthesized DNA; (3) normal advancement of the original replication fork; (4) rate of DNA replication equivalent to in vivo; and (5) expected phenotypic behaviour of temperature-sensitive dna mutants. Systems in Escherichia coli, a membrane-DNA fraction1, an agar-embedded cell lysate2 and toluene-treated cells3 have met two or three of the requirements. Several laboratories have also reported the expected behaviour of ts-dna E. coli mutants in toluenized cells3–5. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |