An Improved Method for Preparing Anti-B Lymphocyte Serum |
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| Authors: | JOHN E. NIEDERHUBER |
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| Affiliation: | 1.Division of Immunobiology,Karolinska Institute, Wallenberglaboratory Lilla Freskati,Stockholm;2.Section of General Surgery,University of Michigan Medical Center,Ann Arbor |
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| Abstract: | THE preparation of a heterologous antiserum specific to the bursa equivalent (B) lymphoid cells of the mouse requires extensive absorption with mouse tissues, especially thymocytes, to obtain specificity. The cell surface antigen(s) against which the serum reacts has been termed mouse specific bone marrow derived lymphocyte antigen(s) (MBLA)1. The specificity of anti-MBLA for the B lymphoid population has been demonstrated by cytotoxicity tests, by its ability to inhibit a portion of antigen binding cells and by adoptive immune responses of antiserum purified lymphoid cells (refs. 1 and 2 and unpublished results of E. Möller and myself). |
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