Structural Changes in Membranes of Synchronized Cells demonstrated by Freeze-cleavage |
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Authors: | ROBERT E. SCOTT RAYMOND L. CARTER WILLIAM R. KIDWELL |
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Affiliation: | 1.Laboratory of Experimental Pathology,National Institute of Arthritis and Metabolic Disease, National Cancer Institute, National Institutes of Health,Bethesda;2.Laboratory of Biochemistry,National Cancer Institute, National Institutes of Health,Bethesda |
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Abstract: | FREEZE-CLEAVAGE is a new technique for studying the ultra-structure· of biological membranes, which fractures cell membranes in half, exposing two intramembranous fracture faces1–3: the outer fracture face (OFF) and the inner fracture face (IFF). These fracture faces are partially covered with 70 Å globular particles which are thought to be unique structural components of cell membranes, formed by the association of membrane glycoproteins and lipids4. The 70 Å particles are dynamic structures and rapidly increase in density in the membranes of lymphocytes following exposure to mitogenic plant proteins (Scott and Marchesi, unpublished work). |
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