Prostaglandin Biosynthesis from Endogenous Precursors in Rabbit Kidney |
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Authors: | KEITH CROWSHAW |
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Institution: | 1.Departments of Internal Medicine and Biochemistry,Saint Louis University School of Medicine Saint Louis,Missouri;2.Biochemistry Department,ARC Institute of Animal Physiology,Babraham, Cambridge |
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Abstract: | THIS report describes the biosynthesis of the naturally occurring renal prostaglandins E2 (PGE2) and F2α (PGF2α)1,2 by homogenates and slices of rabbit renal medulla, from endogenous precursors. I have confirmed that rabbit renal cortex contains little prostaglandin and cannot synthesize them from endogenous lipids3. Hamberg has reported that arachidonic acid, which is converted to PGE2 and PGF2α by enzymes present in ram seminal vesicles4, can be efficiently converted to PGE2 and PGF2α by homogenates of rabbit renal medulla3. I have now confirmed that arachidonic acid, added to such medullary homogenates, can increase the quantities of prostaglandins synthesized. There was no evidence that the major prostaglandin biosynthesized, PGE2, was further metabolized to inactive products. |
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