Different substrates influence the expression of intermediate filaments and the deposition of basement membrane proteins |
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Authors: | Daniel S Liscia Amelia Bernardi Bona Griselli Alberto P M Cappa |
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Institution: | (1) Department of Pathological Anatomy, Ospedale San Giovanni, Torino, Italy;(2) Present address: Laboratory of Tumor Immunology and Biology, National Cancer Institute, National Institutes of Health, 20892 Bethesda, MD |
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Abstract: | Summary A primary culture of serous cystadenocarcinoma of the ovary was used to study the expression of intermediate filament proteins
and the deposition of basal lamina proteins. It was found that cells grown on type I and IV collagens or in collagen gels
failed to express vimentin, which was readily demonstrable in cultures of the same cells grown on plastic or glass. Furthermore
cells grown in collagen gels formed colonies demonstrating a cystic architecture Unlike what is commonly observed on glass
or plastic where laminin and fibronectin are deposited as disorganized fibrils in the extracellular space, in or on collagen
these proteins appear solely at the interface between the epithelial cells and matrix. The results suggest that the extracellular
matrix influences the cytoskeletal organization of the intermediate filaments and determines cell polarity. They confirm that
collagen substrates permit epithelial cell cultures to progress toward a more differentiated state.
Supported by grants from the Italian Assciation for Cancer Research (AIRC). |
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Keywords: | ovarian carcinoma collagen intermediate filaments |
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