小麦分子与细胞遗传学研讨会论文摘要2000年4月22～28日

培育磷高效小麦品种的遗传学与生理学基础 李振声 （中国科学院遗传研究所植物细胞与染色体工程国家重点实验室，北京 100101） 　　关键词：磷高效；小麦；生理基础 　　中图分类号：Q943，Q945　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0041-01 Genetic and Physiological basis for breeding Phosphorus Nutrient Efficient Wheat Varieties LI Zhen-sheng (The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101, China) 用于小麦染色体工程的蓝粒小麦单体系列材料的创制 李振声 （中国科学院遗传研究所植物细胞与染色体工程国家重点实验室） 关键词：染色体工程；蓝粒小麦 中图分类号：Q943　　　文献标识码：A　　　文章编号：0253-9772(2001)-01- Establishment of a Set of blue Grained Wheat Monosomic Lines for Wheat Chromosome engineering studies LI Zhen-sheng (The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101, China) 禾本科植物大小基因组间在基因密度上的共线性与保守性 Beat Keller (Institute of Plant Biology, University of Zürich Zollikerstrasse 107, CH-8008 Zürich, Switzerland) 关键词：禾本科植物；基因组；基因密度；共线性 中图分类号：Q78　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0042-02 Micro-colinearity and Conservation of High Gene Density in Small and Large Grass Genomes Beat Keller (Institute of Plant Biology, University of Zürich Zollikerstrasse 107, CH-8008 Zürich, Switzerland) 利用栽培一粒小麦的BAC文库精细定位小麦基因 Beat Keller (Institute of Plant Biology, University of Zürich Zollikerstrasse 107, CH-8008 Zürich, Switzerland) 关键词：一粒小麦；BAC文库；小麦基因 中图分类号：Q75　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0043-02 High Resolution Mapping of Wheat Genes Using a Triticum monococcum BAC Library Beat Keller (Institute of Plant Biology, University of Zürich Zollikerstrasse 107, CH-8008 Zürich, Switzerland) 图位克隆小麦抗叶锈基因Lr1 凌宏清，Beat Keller (Institute of Plant Biology, University of Zurich, Zollikerstrasse 107. CH-8008 Zurich, Switzerland) 关键词：图位克隆；抗叶锈；小麦 中图分类号： Q343.1　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0044-01 Towards map-based cloning of the leaf rust disease resistance gene Lr1 in wheat LING Hong-qing,Beat Keller (Institute of Plant Biology, University of Zurich, Zollikerstrasse 107. CH-8008 Zurich, Switzerland) 认识和改良中国小麦蛋白质量的遗传基础：策略与现有的研究 王道文，曲乐庆，贾　旭，张相岐，万永芳，李振声 （中国科学院遗传研究所植物细胞与染色体工程国家重点实验室，北京 100101） 关键词：小麦；蛋白质；遗传基础 中图分类号：Q512.1.032　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0045-01 Understanding and Manipulating the Genetic Basis of Protein Quality in Chinese Wheat:Strategies and Current Experiments WANG Dao-wen, QU Le-qing , JIA Xu , ZHANG Xiang-qi, WAN Yong-fang, LI Zhen-sheng (The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics, the Chinese Academy of Sciences, Beijing 100101, China) 比较遗传学研究在认识禾本科植物基因组与基因功能中的应用价值 Mike Gale，Katrien Devos，and Graham Moore (John Innes Centre, Norwich, Norwich Research Park,Colney,Norwich.UK) 关键词：比较遗传学；基因组；基因功能 中图分类号：Q943　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0046-01 Cereal Comparative Genetics-research Opportunities Mike Gale，Katrien Devos，and Graham Moore (John Innes Centre, Norwich, Norwich Research Park,Colney,Norwich.UK) 小麦与环境互作的遗传学基础及其在提高小麦产量中的作用 J W Snape (John Innes Centre, Norwich Research Park, Colney, Norwich,UK) 关键词：小麦；环境互作；遗传学 中图分类号： S521.1　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0046-02 The Genetics of Adaptation in Wheat and Its Role in Maximising Yield Potential J W Snape (John Innes Centre, Norwich Research Park, Colney, Norwich,UK) John Innes中心禾本科植物系所从事的研究： 为认识小麦的生物学而努力 J W Snape (John Innes Centre, Norwich Research Park, Colney, Norwich, UK) 关键词：小麦；禾本科植物 中图分类号：S512.F　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0048-02 Towards Understanding the Biology of wheat:Work in the Cereals Research Department at the John Innes Centre, Norwich, UK J W Snape (John Innes Centre, Norwich Research Park, Colney, Norwich, UK) 杀配子染色体的作用机制及其应用 Takashi Endo (Laboratory of Genetics,Faculty of Agriculture, kyoto University Kyoto, 606,Japan) 关键词：杀配子；染色体；作用机制 中图分类号：Q343　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0049-01 Action and application of gametocidal chromosomes Takashi Endo (Laboratory of Genetics Faculty of Agriculture, kyoto University Kyoto, 606, Japan) 利用杀配子染色体2C诱导大麦染色体产生结构变异 施芳 (Laboratory of Genetics, Faculty of Agriculture, kyoto University Kyoto, 606, Japan) 关键词：杀配子；染色体；大麦 中图分类号：Q343　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0049-02 Generation of structural changes in barley chromosomes by the gametocidal chromosome 2C SHI Fang (Laboratory of Genetics, Faculty of Agriculture, kyoto UniversityKyoto,606,Japan) 小偃麦附加系Z1和Z2中外源染色体2Ai-2的结构组成 张增燕，辛志勇，陈　孝 (中国农业科学院作物育种栽培研究所，北京 100081) 关键词：小偃麦；附加系；染色体 中图分类号：Q343.2　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0050-02 Structural Organization of an Alien Group 2 Chromosome (2Ai-2）in Wheat-Thinopyum intermedium Addition Lines Z1 and Z2 ZHANG Zeng-yan, XIN Zhi-yong, CHEN Xiao (Key Lab of Crop Genetics ＆ Breeding of Ministry of Agriculture， Institute of Crop Breeding and Cultivation，CAAS，Beijing 100081, China) 　　The barley yellow dwarf virus（BYDV）resistance lines of Z1 and Z2 were derived from Zhong 5， a partial amphiploid resulted from the cross between Triticum aestivum（wheat） and Thinopyrum intermedium. Genomic in situ hybridization （GISH） was used to analyze the chromosome constitution of Zhong 5 by using genomic DNA of Pseudoregneria strigosa(StSt，2n＝14）as the probe. The GISH results showed that zhong 5 contains 42 wheat chromosomes and l4 Th.intermedium chromosomes composed of 4 St, 4 Js，4 St-J translocation and 2 St-Js Robertsonian translocation chromosomes. The chromosome constitution of Z1 and Z2 was analyzed by GISH using genomic DNA probes from Th.intermedium and Ps.Strigosa. The GISH results indicated that both Z1 and Z2 possess 42 wheat chromosomes and 2 Th.intermedium chromosomes that were identical to a pair of St-J translocation chromosomes in Zhong 5. The Th.intermedium chromosomes，designated as 2Ai-2 chromosome derived from Zhong 5，mostly belong to the St genome except the middle region (about one third of the long arm) belonging to the E（J）genome. A detailed RFLP analysis was conducted for Z1，Z2 and their parents，St and E (J) genomes. The results of RFLP analyses demonstrated that the Th.intermedium chromosomes（2Ai-2，St-J）in Z1 and Z2 are extensively homologous to the Wheat group 2 chromosomes. The results of RFLP analyses on the genome composition of the 2Ai-2 chromosome were in agreement with the GISH results. Presence of psr 928 on 2AS and 2DS but absence on 2Ai-2S suggests some internal structural differences between 2Ai-2 and the wheat group 2 chromosomes. Some RFLP markers specific to the 2Ai-2 chromosome were identified and may be effectively used to select translocation lines with small segment of the 2Ai-2 chromosome and to localize the BYDV resistance gene in wheat background. Key words：wheat；Thinopyrum intermedium；barley yellow dwarf virus （BYDV）；genomic in situ hybridization （GISH）；RFLP；homoeologous group 2 抗大麦黄矮病的小偃麦易位系的创制与鉴定 辛志勇，张增燕，陈　孝，林志珊 (中国农业科学院作物育种载培研究所，北京 100081) 关键词：大麦；小偃麦；易位系 中图分类号：QS512　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0051-02 Development and Characterization of Common wheat-Thinopyrum intermedium Translocation Lines with Resistance to Barley Yellow Dwarf Virus XIN Zhi-yong,ZHANG Zeng-yan,CHEN Xiao,LIN Zhi-shan, MA You-zhi，XU Hui-jun，XU Qiong-fang，DU Li-pu (Key Lab of Crop Genetics＆Breeding of Agriculture Ministry， Institute of Crop Breeding and Cultivation，CAAS，Beijing，100081，China) 　　Barley yellow dwarf virus（BYDV），vectored by several aphid species，is the most significant viral pathogen of wheat and other grain cereals．Significant economic losses resulting from BYDV in wheat，barley and oats have been reported in many countries．The most economic means of controlling BYDV is to develop wheat varieties with resistance to BYDV. So far no BYDV resistance has been described in wheat collections except one gene in some cultivars tolerant to BYDV. However，Thinopyrum intermedium，two octoploids Zhong 4 awnless and TAF46，and the disomic addition lines，L1，Z1，Z2 and Z6 all showed resistance to BYDV. We developed several wheat-Th.Intermedium translocation lines, Yw642, Yw443 and Yw243 etc., showing good BYDV resistance from L1 by inducing homologous pairing using CS Ph1 mutant. It was found that their BYDV resistance was controlled by a single dominant gene. Characterization of these wheat lines was carried out by GISH and RFLP analysis. The results of GISH showed that the lines, Yw642, Yw443 and Yw243 etc., were homozygous wheat-Th.intermedium translocation lines containing 20 pairs of wheat chromosomes and 1 pair of wheat-Th.intermedium translocation chromosomes，in which the chromosome segments of Th intermedium were transferred to the distal end of a pair of wheat chromosomes. RFLP analysis indicated that the translocation chromosome of the wheat lines was T7DS*7DL-7XL translocation. The breakpoint of translocation is located on the distal end of 7DL，between Xpsr965 and Xpsr680,about 90-99 cM from the centromere. The BYDV gene is located on the distal end of 7XL around Xpsr680，Xpsr687 and Xwg380．The RFLP markers of psr680，psr687 and wg380 co-segregated with the BYDV resistance and could be used for marker-assisted selection（MAS）in wheat breeding program for BYDV resistance. Key words:Thinopyrum intermedium,BYDV，disease resistance， translocation, GISH, RFLP, homoeologous group 7 多枝赖草DNA导入小麦引起重要农艺性状变化及相应的分子证据 李维琪，等 (中国科学院新疆化学研究所，乌鲁木齐 830000) 关键词：小麦；多枝赖草DNA；农艺性状 中图分类号：Q　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0052-02 Important Agronomic trait Changes in Wheat Caused by Introduction of Leymus racemoses DNA and Some Molecular Proofs LI Wei-qi，ZHAO Min-an，LIU Yun-ying，MIAO Jun，LIU Min (Xinjiang Institute of Chemistry, The Chinese Academy of Sciences, Urumqi, 830011) QI Jia-hua，ZHANG Mao-yin，WANG Zi-xia，Haizigoli Yang Ke-rui (Institute of Nuclear and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, 830000) 利用异源双代换系杂交产生染色体易位系 贾　旭 (中国科学院遗传研究所植物细胞与染色体工程国家重点实验室，北京 100101) 关键词：双代换系；染色体；易位系 中图分类号：Q343　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0053-01 Production of Chromosome Translocation via Crossing Two Different Alien Substitution Lines in Wheat JIA Xu (The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101, China) 过去50年中中国小麦品种在Glu-A1, Glu-B1 和Glu-D1位点上等位基因的变化 张学勇，董玉琛 (中国农业科学院品种资源研究所，北京 100081，China) 关键词：小麦；基因 中图分类号： Q943　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0053-02 Allelic variation of Glu-A1, Glu-B1 and Glu-D1 in Chinese Commercial wheat Varieties in the Last 50 Years ZHANG Xue-yong，DONG Yu-cheng (Institute of Crop Germ plasm Resource,CAAS,Beijing 100081，China) 化学杀雄剂导致小麦雄性不育分子机制的初步研究 张爱民，等 (中国农业大学植物遗传育种系，北京 100094，China) 关键词：小麦；杀雄剂；雄性不育；分子机制 中图分类号：Q　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0054-02 Preliminary Study of Molecular Basis of Male Sterility Induced by Chemical Hybridizing Agent in Wheat (Tritium aestivum) ZHANG Ai-min，LIU Dong-cheng (Dept.of Plant Genetics and Breeding, China Agri. Uni.,Beijing 100094,China) 利用小偃麦附加系对Agropyron elongatum生化标记进行染色体定位 刘树兵，等 (山东农业大学农学系，泰安　271018) 关键词：小偃麦；附加系；生化标记 中图分类号：Q343　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0055-02 Chromosomal Location of Agropyron elongatum Markers in Wheat-Agropyron elongatum (2n=14) Addition Lines LIU Shu-bing1, WANG Hong-gang1, ZHOU Rong-hua2，JIA Ji-zeng2 (1.Agronmy Dept., Shandong Agricultural University, Taian 271018，China; 2.Institute of Crop germplasm Resource, CAAS, Beijing, 100081,China) 　　Isoelectric focusing (IFF) technique was used to locate biochemical loci in Agropyron elongatum by using wheat-Agropy-ron elongatum addition lines. There were six loci being located initially in all. The structural genes of Est-E5 and Est-E8 were located in 3EL, β-Amy-1 in 4EL, Per-E1 in 7E, and Per-E4 in 5E. The α-Amy-E1 was relocated in 6EL. Chromosome location of these genes provide evidence of homoeology between wheat groups 3, 4, 6 and Agropyron elongatum chromosome 3E, 4E, 6E, respectively. It also indicated that chromosome rearrangement probably took place between 1E and 7E chromosomes during the evolution of the E genome. Key words:chromosome location, biochemical mark, isoelectric focusing, addition lines 小麦春化相关基因的分子克隆与功能分析 种　康，许智宏，谭克辉 (中国科学院植物研究所，北京 100093) 关键词：小麦；基因；分子克隆 中图分类号：S512.1　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0056-01 Molecular Cloning and Functional Analysis of Vernalization Related Genes in Wheat CHONG Kang, XU Zhi-hong, TAN Ke-hui (Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China) 中字系列小偃麦遗传材料的培育、遗传分析和利用 孙善澄，白建荣 (山西农业科学院作物遗传研究所，太原 030031) 关键词：小偃麦；遗传分析 中图分类号：Q　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0056-02 Breeding, Genetic Analysis and Utilization of “Zhong Series” Varieties of Trielytrigia SUN Shan-cheng,BAI Jian-rong (Institute of Crop Genetics. Shanxi Academy of Agricultural Sciences, Taiyuan 030031，China) 紫外线诱导小麦和长穗偃麦草体细胞融合产生可育株及其后代的细胞遗传学分析 夏光敏，周爱芬，等 (山东大学生命科学院，济南 250100) 关键词：小麦；长穗偃麦草；细胞融合 中图分类号：Q343　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0057-02 Production of Fertile Hybrid Plants Via UV Fusion between Triticum aestivum and Agropyron elongatum and Cytogenetic Analysis of the Resulted Progenies XIA Guang-min,XIANG Feng-ning, ZHOU Ai-fen, LI Zhong-yi,CHEN Hui-min (School of Life Sciences, Shandong University, Jinan 250100,China; *　CSIRO Division of Plant Industry, G.P.O.1600, Australia) 　　Protoplasts of common wheat (Triticum aestivum L.2n=42) cv. Jinan 177 were fused with ultraviolet (UV) irradiated protoplasts of Agropyron elongatum(2n=70) via a PEG method. Sterile hybrid plants were regenerated from the fused product and their ovaries were induced to form calli.From the resulted callus tissue, green plants were differentiated. These somaclonal plants (SF0) were investigated using chromosome and isozyme analysis. The results showed that they contained chromosomes from both donors (I.e.,Triticum aestivum and Agropyron elongatum). Two of the SF0 plants grew to maturity and set seeds. The analysis of phenotype, chromosome constitution, isozyme pattern and RAPD polymorphism of the F1 plants confirmed their hybrid nature. Collectively, these results demonstrated that fertile hybrid plants could be produced from the procedure described above. Three different phenotypes were observed in F2 progenies. The type I and II plants had higher stalks (average 75～85 cm) and big ears and grains, but plants of the former phenotype possessed fewer tillers. Type III plants had short stalks (average 55 cm) but possessed high ability of tillering. Cytogenetical analysis of F1 plants and their successive generations showed that in F1 to F3 generations the chromosome numbers of root tip cells varied in the range of 36～44, and many cells contained 1-4 micro-chromosomes (mc). In PMC MI stage of the F2 plants, the chromosome configuration was mainly 17II-22II with 1-4 additional micro-chromosomes. Comparing to F2, more chromosome configuration of 20II -21II occurred in F3, and over 70% of cells had the chromosome configurations of 21II+1-2 mcs. A large population of the different hybrid lines have been obtained through propagation and selection in successive generations. Their agronomic traits have been studied and will be reported in a separate paper. Key words:Triticum aestivum;Agropyron elongatum;Asymmetric somatic hybridization; Fertile hybrid plants; Cytogenetic analysis 普通小麦F1杂种Glu-1基因表达 过程中的共显性，基因组互作和剂量效应 潘幸来，等 (山西农业科学院棉花研究所，运城 044000) 关键词：小麦；基因表达；基因组 中图分类号：S512.1.035　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0058-01 Evidence for Co-dominance,Genome Interaction and Dosage Effects in Glu-1 Gene Expression in F1 Seeds of Common Wheat (Triticum aestivum L.) PAN Xing-lai, PAN Qian-ying,ZHANG Gui-yuan,WANG Yong-jie,XIE San-gang (Cotton Research Insritute,Shanxi Agri.Sci.Academy,Yuncheng,Shanxi,China,044000) WANG Dao-wen (State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics, Chinese Academy of Sciences, Beijing 100101) PAN Deng-kui (Shanxi Agriculture University, Taigu,Shanxi 030801，China) ph1b基因对簇毛麦遗传物质导入普通小麦的影响 陈　静，等 (中国科学院成都生物研究所，成都 610041) 关键词：ph1b基因；小麦；簇毛麦 中图分类号：Q　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0058-02 Effect of ph1b gene on direct genetic transfer from Haynaldia villosa to Triticum aestivum CHEN Jing,DENG Guang-bing, ZHANG Xiao-pin, MA Xin-rong, YU Mao-qun (Chengdu Institute of Biology, Chinese Academy of Sciences, 610041，China) 　　Hybrid plants between Triticum aestivum var.“Chinese Spring”(CS), its ph1b mutant (CSph1b) and Haynaldia villosa were obtained by immature embryo culture. After selfing, the two types of hybrids showed seed setting rates of 6.67% and 6.25%, respectively. The analysis of chromosome pairing behaviors at meiotic metaphase I showed that, on the average, only 1.61 chromosomes could form bivalent and trivalent in each PMC of the hybrid F1 CS×H. Villosa, with the configuration 2n=28=26.39I+0.79II+0.007III. However, in the hybrid F1 CS ph1b×H. Villosa, 14.43 chromosomes per PMC were involved in bivalent and multivalent formation, with the chromosome configuration of 2n=28=13.55I+5.95II+0.55III=0.22IV, and, in over 56% of the PMCs, 1-4 multivalents (trivalents and quadrivalents) were produced. The observation of meiotic chromosome pairing by using genomic fluorescent in situ hybridization (GISH) revealed three types of chromosomal associations: wheat-wheat, wheat-H. Villosa and H. Villosa-H. Villosa in PMCs for ‘CS ph1b×H.villosa’F1 hybrid. The seed set of the backcross of the‘CS ph1b×H.villosa’F1 with CS was 6.67% and that of‘CS×H.villosa’F1 with CS or CS ph1b was only 0.45%. The chromosome number of BC1 plants varied from 48 to 72. Robertsonian translocation chromosomes consisting of chromosome arms from wheat and H.villosa were detected by mitosis GISH in the BC1 plants from the backcross of‘CSph1b×H.villosa’to CS ph1b. These results led to the conclusion that the ph1b gene induced a higher level of homoeologous chromosome pairing between common wheat and H.villosa.The transfer of desirable genes from H.villosa to common wheat may be facilitated by using the ph1b gene. Key words:genetic transfer, GISH,Haynaldia villosa；ph1b gene；homoeologous pairing；Triticum aestivum 利用分子细胞遗传学方法向小麦中转移和富积优异外源基因 陈佩度 (南京农业大学细胞遗传研究所，南京 210095) 关键词：小麦；细胞遗传学；外源基因 中图分类号：Q343.1　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0059-02 Utilization of Molecular Cytogenetics Technology in Transferring and Pyramiding Useful Alien Genes into Common Wheat CHEN Pei-du (Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095,China) 小麦品种复壮30中与抗白粉病基因连锁的一个RAPD标记 王立新，等 (北京市农业科学院植物细胞工程实验室，北京 100089) 关键词：小麦；抗白粉病；基因；RAPD标记 中图分类号：Q343.1　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0060-01 A RAPD Marker Linked to the Resistance Gene to Powdery Mildew in Wheat Variety-Fu Zhuang 30 WANG Li-xin, SU Ai-lian, XU Min-xin,WANG Xiu-qin,Peter.P.Ueng*,JIA Ji-zheng** (Beijing Plant Cell Bioengineering Laboratory, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089,China； *　Molecular Plant Pathology Laboratory, USDA-ARS, MD 20705,US **　Key Laboratory of Crop Germplasm and Biotechnology, Institute of Crop Germplasm Resource,China Academy of Agriculture Sciences，Beijing 100081,China) 小麦和簇毛麦体细胞可育杂种植株的产生 周爱芬，夏光敏，等 (山东大学生命科学学院，济南 250100) 关键词：小麦；簇毛麦；体细胞 中图分类号：S512.035.1　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0060-02 Production of Fertile Somatic Hybrid Plants Between Triticum Aestivum and Haynaldia villosa ZHOU Ai-fen1，XIA Guang-min1，ZHANG Xiang-qi2，CHEN Hui-min，HU Han2 (1.Life Science School, Shandong University, Jinan 250100，China； 2.Institute of Genetics, Chinese Academy of Science, Beijing 100101，China) 小麦基因组中外源染色体片段的检测和小麦基因分子标记的建立 石锐 (哈尔滨师范大学生物系，150080) 关键词：小麦；外源染色体；分子标记 中图分类号：Q75　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0061-01 Studies on the Detection of Alien Chromosome Fragments and Obtainment of Molecular Markers Linked to Specific Genes in Wheat SHI Rui (Department of Biology,Harbin Normal University,Harbin,Heilongjiang Province 150080，China) 小麦春化发育机制的初步研究 尹　军,等 (山西农业大学，太谷　030801) 关键词：小麦；春化；发育机制 中图分类号：Q344　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0062-01 Preliminary Study on the Mechanism of the Vernalization Development in Wheat YIN Jun，REN Jiang-ping，DONG Ai-xiang （Shanxi Agricultural University，Taigu 030801,China） 非Robertsonian类型小黑麦易位系的研究 胡　含 (中国科学院遗传研究所植物细胞与染色体工程国家重点实验室，北京 100101) 关键词：小黑麦；易位系 中图分类号：Q343　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0062-02 About non-Robertsonian wheat-rye chromosome translocation lines HU Han (State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics CAS,Beijing 100101,China) 小麦6B染色体的微切割与其区域特异性DNA文库的构建 周奕华，王　槐，陈正华 (中国科学院遗传研究所，北京 100101) 关键词：小麦；微切割；染色体；DNA 中图分类号：Q343　　　文献标识码：A　　　文章编号：0253-9772(2000)-01-0063-01 Micro-dissection of Wheat Chromosome 6B and Construction of Its Region Specific DNA Libraries ZHOU Yi-hua,WANG Huai,CHEN Zheng-hua (Institute of Genetics,Chinese Academy of Sciences, Beijing 100101，China) 组织培养诱导外源染色体发生结构 变异及其在小麦易位系创制中的利用 李洪杰1，贾　旭1，楚成才2 (1.中国科学院遗传研究所植物细胞与染色体工程国家重点实验室，北京 100101；2.中国科学院植物研究所，北京 100093) 关键词：小麦；染色体；结构变异；易位系 中图分类号：Q343　　　文献标识码：A　　　文章编号：0253-9772(2001)-01-0064-01 Structural Changes of Alien Chromosomes Arising from Tissue Culture and Their Exploitation in Producing Novel Translocation Wheat Lines LI Hong-Jlie1,2，JIA Xu1，CHU Cheng-chai2 (1.The State Key Laboratory of Plant cell and Chromosome Engineering, Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101, China； 2.Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China)