Abnormal erythrocyte membrane phospholipid organisation in chronic myeloid leukaemia |
| |
Authors: | A Kumar S Daniel S S Agarwal C M Gupta |
| |
Institution: | (1) Division of Biochemistry, Central Drug Research Institute, 226 001 Lucknow, India;(2) Department of Medicine, King George’s Medical College, 226 003 Lucknow, India |
| |
Abstract: | The membrane phospholipid organisation in the red cells of humans suffering from chronic myeloid leukaemia has been analysed
using the amino-group labelling reagent trinitrobenzenesulphonic acid and the fluid-sensing fluorophore, Merocyanine 540.
Unlike the normal human erythrocytes, trinitrobenzenesulphonic acid in intact chronic myeloid leukaemia erythrocytes modified
about 30% phosphatidylserine, under controlled conditions. Also, the chronic myeloid laukaemia red cells, but not the normal
cells, were found to bind the fluorescent dye Merocyanine 540. These results demonstrate that loss of the transmembrane phospholipid
asymmetry in chronic myeloid leukaemia erythrocytes is accompanied by an enhancement in the outer surface fluidity and, therefore,
suggest that the red cells membrane phase-state asymmetry originates probably from the asymmetric arrangements of phospholipids
across the membrane bilayer. |
| |
Keywords: | Phospholipid asymmetry erythrocytes Phase-state asymmetry chronic myeloid leukaemia |
本文献已被 SpringerLink 等数据库收录! |
|