Esre: a novel essential non-coding RNA in Escherichia coli |
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| Authors: | Chen Zhichao Wang Yi Li Yarong Li Yue Fu Nan Ye Jiang Zhang Huizhan |
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| Institution: | State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China. |
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| Abstract: | YigP gene (GeneID: 948915) locates between ubiquinone biosynthetic genes ubiE and ubiB in Escherichia coli. GeneBank annotates yigP as a putative protein-coding gene. In this study, we found a new essential sRNA gene, esre, locates within the region of yigP. The E. coli strain with inactive esre must rely on a complementary plasmid to survive. Moreover, RACE experiments showed esre encodes an RNA molecule of 252 nt. Further experiments revealed esre gene is immune to frame shift mutations and the function of esre depends mostly on the RNA secondary structure, which are typical traits of sRNA. Since it is difficult to predict the target of an essential sRNA, more research is needed to reveal the function and mechanism of esre. |
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