Formation of plantlets through somatic embryogeny in the Himalayan blue poppy,Meconopsis simplicifolia (Papaveraceae) |
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Authors: | I M Sulaiman N S Rangaswamy C R Babu |
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Institution: | (1) Department of Botany, University of Delhi, 110007 Delhi, India |
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Abstract: | Summary Exuberant and subculturable calli could be induced from only hypocotyl and leaf segments of ca 4-month-old seedlings of Meconopsis simplicifolia cultured on Murashige & Skoog's medium supplemented with 10–6M kinetin + 10–5M -naphthalene acetic acid. Suspension cultures were initiated from the calli in a similar medium but with 10–5M 2,4-dichlorophenoxy acetic acid in place of -naphthalene acetic acid. In ca 80% of the suspension cultures somatic embryos differentiated freely (80–85%) as well as on the surface of small clumps of tissue (15–20%). Somatic embryos that developed beyond heart-shaped stage were transferred to agar-solidified Murashige & Skoog's medium free of growth substances. When maintained in 10 h light and 14 h dark the somatic embryos developed into plantlets bearing cauline leaves. From seed sowing to raising normal plantlets via callus required 28 weeks; on average 80 plantlets were obtained from one explant in three passages.Abbreviations 2,4-D
2,4-dichlorophenoxy acetic acid
- FAA
formalin-acetic acid-alcohol
- GA3
gibberellic acid
- Kn
kinetin
- MS
Murashige & Skoog's medium (Murashige and Skoog 1962)
- NAA
-naphthalene acetic acid |
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