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An age-dependent proliferation is involved in the postnatal development of interstitial cells of Cajal in the small intestine of mice
Authors:Feng Mei  Jiang Zhu  Sheng Guo  De-shan Zhou  Juan Han  Bin Yu  Shi-feng Li  Zhong-yong Jiang  Cheng-jie Xiong
Institution:(1) Department of Histology and Embryology, Third Military Medical University, 400038 Chongqing, China;(2) Department of Pathology, Southwest Hospital, Third Military Medical University, 400038 Chongqing, China;(3) Department of Immunology, Third Military Medical University, 400038 Chongqing, China;(4) Department of Histology and Embryology, Capital University of Medical Sciences, 100054 Beijing, China
Abstract:This paper aimed at investigating the alterations in interstitial cells of Cajal (ICCs) in the murine small intestine from 0-day to 56-day post-partum (P0–P56) by immunohistochemistry. The Kit+ ICCs, which were situated around myenteric nerve plexus (ICC-MY) formed a loose cellular network at P0 which changed into an intact one before P32. The density of ICC-MY increased from P0 to P12, and then decreased until P32. In contrast, the estimated total amount increased more than 15-fold at P32 than that at P0. Some Kit+/BrdU+ cells were observed at 24 h after one BrdU injection to the different-aged mice, and the number decreased from P2 to P24 and vanished at P32. Actually a few Kit+/BrdU+ cells can be observed at 1 h after one BrdU injection at P10, and the amount doubled at 24 h along with paired Kit+/BrdU+ cells. A number of BrdU+ ICCs were also labeled with CD34, CD44 and insulin-like growth factor I receptor. About 65% ICCs were BrdU+ at P32 after daily BrdU injection from P0. Our results indicate that an age-dependent proliferation is involved in the postnatal development of ICC-MY which increase greatly in cell numbers and proliferative ICCs may originate from ICCs progenitor cells. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. Feng Mei and Jiang Zhu have contributed equally to this work.
Keywords:Proliferation  Kit  BrdU  Precursor  Progenitor
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