大熊猫酸性核糖体磷酸蛋白P1 基因cDNA 克隆及序列分析

运用RT-PCR 技术,从大熊猫的肌肉组织总RNA 中成功克隆了酸性核糖体磷酸蛋白P1 (RPLP1)基因的表达序列,并对其进行了测序及初步分析。结果表明:大熊猫RPLP1 基因的表达序列全长为448 bp,开放阅读框(ORF)为344 bp,编码114 个氨基酸的蛋白质,该蛋白的分子量为11.566 kDa,pI 为4.4,含有3 个酪蛋白激酶Ⅱ磷酸化位点和2 个N - 酰基化位点。进一步分析发现,大熊猫RPLP1 基因的表达序列及其编码的氨基酸序列与已报道的部分哺乳动物具有很高的相似性。

cDNA cloning and sequences analysis of acidic ribosomal phosphoprotein P1 (RPLP1) from giant panda

The cDNA of acidic ribosomal phosphoprotein P1 (RPLP1)was cloned successfully from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology,which was also sequenced,analyzed preliminarily. The result shows that the cDNA fragment cloned is 449 bp in size,and it contains an open reading frame of 344 bp encoding 114 amino acids.The deduced protein sequence shows that the protein is composed of 114 amino acids and its estimated molecular weight is 11.566 kDa with a pI of 4.4. Alignment analysis indicated that the nucleotide sequence and the deduced amino acid sequence are highly conserved to other five species studied,including Homo sapiens,Mus musculus,Rattus norvegicus,Bos taurus and Sus scrofa. The homologies for nucleotide sequences of giant panda PPLP1 to that of these species are 92.4% , 89.8%,89.0% ,91.3% and 87.5%,while the homologies for amino acid sequences are 96.5% ,94.7% ,95.6%, 96.5% and 88.6%. Topology prediction showed there are three Casein kinase II phosphorylation sites and two N-myristoylationsites in the RPLP1 protein of the giant panda.