Molecular Cloning of OSP94: A Significant Biomarker Protein of Hypertensive Human Heart and a Member of HSP110 Family |
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Authors: | John Geraldine Sandana Mala Satoru Takeuchi |
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Institution: | (1) Factory of Takeuchi Nenshi, TAKENEN, 85 NE, Takamatsu, Kahoku, Ishikawa 929 1215, Japan |
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Abstract: | Heat shock proteins (HSPs) are upregulated in response to stress and play a protective function in refolding of cellular proteins.
In hypertension, the heart is a vital organ that requires examination and investigation, and primary induction of HSPs is
predominantly effected. Hypertension results from osmotic imbalance during renin-angiotensin cycle inefficiency. Osmotic stress
protein 94 (OSP94) is a stress protein induced upon osmotic imbalance. It is therefore necessary to analyze its precise role
in the hypertensive heart. We have first reported the cloning and expression of human heart OSP94 followed by an analysis
of gene sequence and protein homology. Directional cloning of OSP94 by PCR amplification yielded a 2.5 kb amplicon and was
cloned into pET-15b. Site-directed mutagenesis was essentially followed. mRNA expression levels were evaluated in correlation
with HSPs. Gene analysis indicated a 2520 bp sequence with an 838-amino acid protein complement. Protein homology revealed
highly conserved sequence similarity among mammalian sequences. Structural predictions of OSP94 protein were also investigated.
OSP94 is therefore recognized as a significant stress protein for investigations in hypertensive heart tissues and is a highly
conserved protein in the HSP110 subfamily.
Sequences deposited: EF 197155, NCBI GenBank Accession No. for OSP94 gene sequence; ABM 69040, NCBI GenPept Accession No.
for OSP94 protein sequence. |
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Keywords: | Hypertension Human heart tissue mRNA expression OSP94 Sequence alignment Sequence analysis |
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