The response of a nitrogen-limited chemostat culture of Escherichia coli B/r to pH and dilution rate steps |
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Authors: | Goochee C F Hatch R T Cadman T W |
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Affiliation: | Department of Chemical and Nuclear Engineering, University of Maryland, College Park, Maryland 20742. |
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Abstract: | The effects of four pH steps and one dilution rate step are described for an ammonia-nitrogen-limited continuous culture of Escherichia coli B/r. Two of the pH steps, 6.06-5.49 and 5.96-5.60, led to prolonged transients in both cell density and rate-limiting nutrient concentration. The other two pH steps, 6.20-5.96 and 5.60-6.20, had almost no effect on the culture. The dilution rate step led to a sharp transition in the steady-state external pyruvate concentration. Monitoring of the external pyruvate concentration for the pH 5.96-5.60 step revealed that the transient phase continued long after the cell density and rate-limiting nutrient concentration returned to steady-state values. The implications for industrial and laboratory fermentations are discussed. |
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