Transport and intracellular localization of cortisol-asialotranscortin complexes in rat liver |
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| Authors: | H Karásková K Bezouska L Stárka R Hampl A T Pikulev M V Sholukh O Táborsky |
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| Affiliation: | 1. SINTEF Industry, New Energy Solutions, Trondheim, Norway;2. SINTEF Industry, Materials Physics, Oslo, Norway;3. SINTEF Industry, Process Metallurgy and Raw Materials, Trondheim, Norway |
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| Abstract: | The influence of desialylation of human transcortin on transport of the transcortin-cortisol complex into the liver cells and its intracellular distribution was investigated in perfused rat liver. Under experimental conditions used the half-time of cortisol in perfusion medium was decreased more than 200 times in presence of asialotranscortin compared to that of native transcortin. Experiments with [3H]cortisol and [131I]asialotranscortin demonstrated a simultaneous uptake of cortisol and asialotranscortin by the hepatocytes. Distribution of [3H]cortisol and [131I]asialotranscortin in subcellular fractions showed the following pathway of cortisol-asialotranscortin complex: cell membrane, lysosomes, cytoplasm. The complex dissociates in lysosomes. |
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