Lactococcus lactis as a cell factory for high-level diacetyl production |
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| Authors: | Hugenholtz J Kleerebezem M Starrenburg M Delcour J de Vos W Hols P |
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| Affiliation: | Wageningen Centre for Food Sciences and NIZO Food Research, 6710 BA Ede, The Netherlands. hugenhol@nizo.nl |
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| Abstract: | We report the engineering of Lactococcus lactis for the efficient conversion of sugar into diacetyl by combining NADH-oxidase overproduction and alpha-acetolactate decarboxylase inactivation. Eighty percent of the carbon flux was found to be rerouted via alpha-acetolactate to the production of diacetyl by preloading the cells with NADH-oxidase before their use as a cell factory. |
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