| 脑微血管内皮细胞条件培养液对星形胶质细胞的影响及通络中药的干预特征 |
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| 引用本文： | 唐卉凌,李澎涛,张玮,潘彦舒,王筠,李卫红,侯金才,张韫. 脑微血管内皮细胞条件培养液对星形胶质细胞的影响及通络中药的干预特征[J]. 现代生物医学进展, 2010, 10(5) |
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| 作者姓名： | 唐卉凌 李澎涛 张玮 潘彦舒 王筠 李卫红 侯金才 张韫 |
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| 作者单位： | 1. 北京中医药大学基础医学院,北京,100029
2. 中日友好医院临床研究所,北京,100029 |
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| 基金项目： | “重大新药创制”科技重大专项-基于脑病治疗的中药复方药理学及药效学评价关键技术研究(2009ZX09502-014); 国家十一五支撑计划-基于脑微血管内皮细胞功能物质发现的脑病药物筛选与效应分析新技术(2006BAI08B05); 教育部科学技术研究重点项目-从内皮-胶质细胞信息网络解析脑络功能及其病理基础(109023) |
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| 摘 要： | 目的:揭示脑微血管内皮细胞生理、病理及通络中药处理后不同状态的培养液对正常星形胶质细胞影响的特征,从细胞间相互作用角度探讨脑微血管内皮细胞与星形胶质细胞的生物学关系,为阐释脑微环境稳定的血脑屏障维护机制以及通络中药通过内皮细胞调节脑内微环境理论假说提供新的证据。方法:制备正常、拟缺血和拟缺血合并通络救脑注射液处理的大鼠脑微血管内皮细胞条件培养液,观察其对星形胶质细胞活性和凋亡率的影响。结果:与正常星形胶质细胞相比,正常内皮细胞条件培养液能够降低正常星形胶质细胞的活性,并促进星形胶质细胞的凋亡;而拟缺血处理的内皮细胞条件培养液能够提高正常星形胶质细胞的活性和凋亡率;拟缺血合并通络药物处理的内皮细胞条件培养液对正常星形胶质细胞的活性有提高作用,并显著降低其凋亡率。结论:三种不同处理方式的内皮细胞条件培养液对正常星形胶质细胞活性和凋亡产生不同的影响,提示不同状态的微血管内皮细胞对脑内微环境产生影响,通络救脑注射液可能通过调节微血管内皮细胞的分泌而对星形胶质细胞发挥作用。
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| 关 键 词： | 脑微血管内皮细胞条件培养液 通络救脑注射液 星形胶质细胞 增殖活性 凋亡率 |
Effect of conditioned mediums of rat brain microvascular endothelial cells on normal astrocyte and influence of Tongluo prescription |
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| TANG Hui-ling,LI Peng-tao,ZHAG Wei,PAN Yan-shu,WANG Jun,LI Wei-hong,HOU Jin-cai,ZHANG Yun. Effect of conditioned mediums of rat brain microvascular endothelial cells on normal astrocyte and influence of Tongluo prescription[J]. Progress in Modern Biomedicine, 2010, 10(5) |
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| Authors: | TANG Hui-ling LI Peng-tao ZHAG Wei PAN Yan-shu WANG Jun LI Wei-hong HOU Jin-cai ZHANG Yun |
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| Affiliation: | TANG Hui-ling1,LI Peng-tao1,ZHANG Wei1,PAN Yan-shu1,WANG Jun 1,LI Wei-hong1,HOU Jin-cai1,ZHANG Yun2 (1 The Preclinical Medicine College of Beijing University of TCM Beijing 100029,China,2 The Clinical Academy of China-Japan Friendship Hospital Beijing 100029,China ) |
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| Abstract: | Objective:To observe the effect of different conditioned mediums of rat brain microvascular endothelial cells on the normal astrocyte,as well as to investigate the interaction between microvascular endothelial cell and astrocyte,and the influence of Tongluo Jiunao injection (TLJN)on astrocyte by affecting endothelial.Methods:The conditioned medium by normal,oxygen-glucose deprivation (OGD) and OGD+TLJN intervened cerebral microvascular endothelia cells of rat were collected after differently treated which w... |
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| Keywords: | Conditioned mediums of rat brain microvascular endothelial cells Tongluo Jiunao injection Astrocyte Activity Apoptosis |
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