Transmembrane currents in frog olfactory cilia |
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Authors: | Steven J. Kleene Robert C. Gesteland |
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Affiliation: | (1) Department of Anatomy and Cell Biology, University of Cincinnati, 45267-0521 Cincinnati, Ohio |
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Abstract: | Summary We have measured transmembrane currents in intact single cilia from frog olfactory receptor neurons. A single cilium on a neuron was sucked into a patch pipette, and a high-resistance seal was formed near the base of the cilium. Action potentials could be induced by applying suction or a voltage ramp to the ciliary membrane. A transient current was seen in some cells on stimulation with odorants. After excision from the cell, most of the cilia showed increased conductance in a bath containing cAMP, indicating that the cytoplasmic face of the ciliary membrane was accessible to the bath. The estimated resistance of a single cilium was surprisingly low. |
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Keywords: | olfaction receptor electrophysiology |
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