Optical mapping and its potential for large-scale sequencing projects. |
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Authors: | C Aston B Mishra D C Schwartz |
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Affiliation: | Wyeth-Ayerst Research, CNS Disorders, Princeton, NJ 08543, USA. astonc01@mcrcr.med.nyu.edu |
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Abstract: | Physical mapping has been rediscovered as an important component of large-scale sequencing projects. Restriction maps provide landmark sequences at defined intervals, and high-resolution restriction maps can be assembled from ensembles of single molecules by optical means. Such optical maps can be constructed from both large-insert clones and genomic DNA, and are used as a scaffold for accurately aligning sequence contigs generated by shotgun sequencing. |
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