Design of a fluorescence polarization assay platform for the study of human Hsp70 |
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| Authors: | Kang Yanlong Taldone Tony Clement Cristina C Fewell Sheara W Aguirre Julia Brodsky Jeffrey L Chiosis Gabriela |
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| Affiliation: | Program in Molecular Pharmacology and Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA. |
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| Abstract: | The 70kDa heat shock proteins (Hsp70) are molecular chaperones that assist in folding of newly synthesized polypeptides, refolding or denaturation of misfolded proteins, and translocation of proteins across biological membranes. In addition, Hsp70 play regulatory roles in signal transduction, cell cycle, and apoptosis. Here, we present a novel assay platform based on fluorescence polarization that is suitable for investigating the yet elusive molecular mechanics of human Hsp70 allosteric regulation. |
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