A quantitative microdensitometric and autoradiographic study of the effect of 4'-demethyl-epipodophyllotoxin-beta-D-thenylidene glucoside (VM-26) on the cell cycle of cultured fibroblasts |
| |
Authors: | V Gotzos B Cappelli-Gotzos and J -M Despond |
| |
Institution: | (1) Institute of Histology and General Embryology, University of Fribourg, Switzerland |
| |
Abstract: | Synopsis The effect of 4 -demethyl-epipodophyllotoxin- -d-thenylidene glucoside (VM-26), a semi-synthetic derivative of podophyllotoxin, on the cell cycle was studied with chick embryo fibroblasts cultivatedin vitro. DNA, RNA and protein content, as well as NADH-diaphorase activity were determined by quantitative microdensitometry and cytofluorometry. The incorporation of 3H]thymidine and 3H]leucine into DNA and proteins were analysed by autoradiography. These metabolic data correlated with morphological observation showed that VM-26 blocks the cell cycle at different moments of its kinetics depending on both the dose and the time exposure. NADH-diaphorase activity is the first to be affected, then biochemical changes (involving the metabolism of RNA and proteins) and morphological alterations (especially of mitochondria) follow. This suggests that VM-26 may act primarily upon the mechanism of respiration of the cell.Dedicated to Professor G. Conti on the occasion of his 60th birthday. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|